In-house validation method for quantification of formaldehyde in silage and feedingstuffs with the use HPLC-DAD technique

An HPLC-DAD method was developed for the determination of formaldehyde in animal feed and silage. The is based on product chemical reaction between 2,4-dinitrophenylhydrazine. A 3 g or silage were extracted with Milli-Q water phosphoric acid next derivative use 2,4-dinitrophenyl- hydrazine acetronitrile solution. extract purified 0.45 µm syringe filters separeted Zorbax Eclipse XDB C18 column detection carried out at 360 nm. Formal- dehyde eluted a mobile phase consisting acetonitrile/water isocratic elution. This provided average recoveries 90.6% to 102.2%, CVs 2.6% 6.4% from 91.3% 108.7% 1.1% 4.1% ranged 50 1000 mg/kg feeds LOD LOQ 1.6 2.6 2.7 5.7 mg/kg, respectively. methodology applied analysis samples collected poultry, pigs cows farms.